Review



goat anti pdx1  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    R&D Systems goat anti pdx1
    Goat Anti Pdx1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 137 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+pdx1/Human+PDX-1%2FIPF1+Antibody/pm41927580-280-25-28
    Average 93 stars, based on 137 article reviews
    goat anti pdx1 - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    other:

    Article Title: Depolymerizing F-actin accelerates the exit from pluripotency to enhance stem cell-derived islet differentiation.
    Article Snippet: The proteins were transferred onto PVDF membrane (1620177, BioRad), blocked in 5% milk (1706404, BioRad) for 1 hour at room temperature, and incubated in primary antibodies in 5% milk overnight at 4°C.

    Article Title: Anti-TNF-α antisense-oligonucleotide-conjugated PLG nanoparticles protect transplanted islets
    Article Snippet: Primary and secondary antibodies used: rat anti-C-peptide (Developmental Studies Hybridoma Bank, University of Iowa, cat. no. GN-ID4-S), mouse anti-NKX6-1 (Developmental Studies Hybridoma Bank, University of Iowa, cat. no. F55A12-S), goat anti-PDX1 (R&D Systems, cat. no. AF2419), anti-mouse Alexa Fluor 488 (Invitrogen, cat. no. A21202), anti-rat Alexa Fluor 405 (Abcam, cat. no. AB175670 ), anti-mouse Alexa Fluor 647 (Invitrogen, cat. no. A31571), and anti-goat Alexa Fluor 647 (Invitrogen, cat. no. A21447).

    Article Title: Integrative single-cell multi-omics profiling of human pancreatic islets identifies T1D-associated genes and regulatory signals
    Article Snippet: Goat Anti-PDX1 , R & D Systems , Cat#AF2419; RRID: AB_355257.

    Paraffin Section:

    Article Title: Human pancreatic organoids derived from pluripotent stem cells recapitulate pancreatic organogenesis
    Article Snippet: .. Primary antibodies used in this study were: goat anti-PDX1 (1:200, R&D Systems, AF2419), mouse anti-NKX6.1 (1:100, DSHB, F55A10), rabbit anti-SOX9 (1:200, EMD Millipore, AB5535), sheep anti-neurogenin3 (1:200, R&D Systems, AF3444), rat anti-C-peptide (1:200, DHSB, GNID4), rabbit anti-glucagon (1:200, Cell Marque, 259R15), mouse anti-chromogranin A (1:200, DHSB, CPTC-CHGA-1), mouse anti-cytokeratin 19 (1:200 for paraffin section, ThermoFisher Scientific, MA515884), rabbit anti-mucin 1 (1:200, Sigma-Aldrich, HPA008855), mouse anti-CPA1 (1:200, OriGene Technologies, TA500053), rabbit anti-GATA4 (1:200, Santa Cruz Biotechnology, sc9053), rabbit anti-trypsin (1:200, ThermoFisher Scientific, PA5106876), rabbit anti-amylase (1:200, Sigma-Aldrich, HPA045394), mouse anti-CDH17 (1:500, Sigma-Aldrich, SAB1403654), rat anti-SOX2 (1:200, R&D Systems, AF2018), rabbit anti-CDX2 (1:200, Cell Marque, 235R16), rabbit anti-claudin 18 (1:200, Sigma-Aldrich, HPA018446), rabbit anti-KI67 (1:200, Cell Marque, 275R14), goat anti-CDH1 (1:400, R&D Systems, AF648), mouse anti-ZO1 (1:200, ThermoFischer Scientific, 339100), rabbit anti-laminin (1:200, Abcam, ab11575), rabbit anti-collagen I (1:200, Abcam, ab34710), rabbit anti-WT1 (1:100, Abcam, ab89901), mouse anti-SMA (1:200, Sigma-Aldrich, A5228), mouse anti-vimentin (1:200, Santa Cruz Biotechnology, sc373717), goat anti-FOXF1 (1:200, R&D Systems, AF4798). .. Secondary antibodies used to visualize proteins included donkey anti-mouse Alexa Fluor 488, 568 and 647 (1:500; ThermoFisher Scientific, A21202, A10037, A31571), donkey anti-goat Alexa Fluor 647 (1:500; Thermo Scientific, A21447), donkey anti-rabbit Alexa-Fluor 568 or 647 (1:500; Thermo Scientific, A10042, A31573), donkey anti-rat 488 (1:500; Thermo Scientific, A21208), and donkey anti-sheep 647 (1:500; Thermo Scientific, A21448).

    Blocking Assay:

    Article Title: Expression of genes involved in thyroid hormone action in human induced pluripotent stem cells during differentiation to insulin-producing cells: Effects of iopanoic acid on differentiation.
    Article Snippet: Dissociated cells were fixed using a Cytofix/Cytoperm Kit (BD Biosciences, Franklin Lakes, NJ, USA) according to the manufacturer’s protocol. .. Primary antibodies were diluted with blocking solution and incubated at 4 ◦C for 16 h. The primary antibodies used for the flow cytometry analysis included goat anti-SOX17 (R&D Systems; AF 1924, 1:200; RRID:AB_355060), mouse anti-HNF4α (Santa Cruz Biotechnology; sc-374229, 1:500; RRID:AB _10989766), goat anti-PDX1 (R&D Systems; AF 2419, 1:200; RRID: AB_355257), mouse anti-NKX6.1 (DSHB, Iowa City, IA, USA; F55A12, 1:100; RRID:AB_532379), rabbit anti-CHGA (Abcam, Cambridge, UK; ab68271, 1:500; RRID:AB_11154750), and rat anti-CPR (DSHB; GN-ID4, 1:200; RRID:AB_2255626). .. Primary antibodies were diluted with blocking solution and incubated at 4 ◦C for 16 h. The primary antibodies used for the flow cytometry analysis included goat anti-SOX17 (R&D Systems; AF 1924, 1:200; RRID:AB_355060), mouse anti-HNF4α (Santa Cruz Biotechnology; sc-374229, 1:500; RRID:AB _10989766), goat anti-PDX1 (R&D Systems; AF 2419, 1:200; RRID: AB_355257), mouse anti-NKX6.1 (DSHB, Iowa City, IA, USA; F55A12, 1:100; RRID:AB_532379), rabbit anti-CHGA (Abcam, Cambridge, UK; ab68271, 1:500; RRID:AB_11154750), and rat anti-CPR (DSHB; GN-ID4, 1:200; RRID:AB_2255626).

    Incubation:

    Article Title: Expression of genes involved in thyroid hormone action in human induced pluripotent stem cells during differentiation to insulin-producing cells: Effects of iopanoic acid on differentiation.
    Article Snippet: Dissociated cells were fixed using a Cytofix/Cytoperm Kit (BD Biosciences, Franklin Lakes, NJ, USA) according to the manufacturer’s protocol. .. Primary antibodies were diluted with blocking solution and incubated at 4 ◦C for 16 h. The primary antibodies used for the flow cytometry analysis included goat anti-SOX17 (R&D Systems; AF 1924, 1:200; RRID:AB_355060), mouse anti-HNF4α (Santa Cruz Biotechnology; sc-374229, 1:500; RRID:AB _10989766), goat anti-PDX1 (R&D Systems; AF 2419, 1:200; RRID: AB_355257), mouse anti-NKX6.1 (DSHB, Iowa City, IA, USA; F55A12, 1:100; RRID:AB_532379), rabbit anti-CHGA (Abcam, Cambridge, UK; ab68271, 1:500; RRID:AB_11154750), and rat anti-CPR (DSHB; GN-ID4, 1:200; RRID:AB_2255626). .. Primary antibodies were diluted with blocking solution and incubated at 4 ◦C for 16 h. The primary antibodies used for the flow cytometry analysis included goat anti-SOX17 (R&D Systems; AF 1924, 1:200; RRID:AB_355060), mouse anti-HNF4α (Santa Cruz Biotechnology; sc-374229, 1:500; RRID:AB _10989766), goat anti-PDX1 (R&D Systems; AF 2419, 1:200; RRID: AB_355257), mouse anti-NKX6.1 (DSHB, Iowa City, IA, USA; F55A12, 1:100; RRID:AB_532379), rabbit anti-CHGA (Abcam, Cambridge, UK; ab68271, 1:500; RRID:AB_11154750), and rat anti-CPR (DSHB; GN-ID4, 1:200; RRID:AB_2255626).

    Flow Cytometry:

    Article Title: Expression of genes involved in thyroid hormone action in human induced pluripotent stem cells during differentiation to insulin-producing cells: Effects of iopanoic acid on differentiation.
    Article Snippet: Dissociated cells were fixed using a Cytofix/Cytoperm Kit (BD Biosciences, Franklin Lakes, NJ, USA) according to the manufacturer’s protocol. .. Primary antibodies were diluted with blocking solution and incubated at 4 ◦C for 16 h. The primary antibodies used for the flow cytometry analysis included goat anti-SOX17 (R&D Systems; AF 1924, 1:200; RRID:AB_355060), mouse anti-HNF4α (Santa Cruz Biotechnology; sc-374229, 1:500; RRID:AB _10989766), goat anti-PDX1 (R&D Systems; AF 2419, 1:200; RRID: AB_355257), mouse anti-NKX6.1 (DSHB, Iowa City, IA, USA; F55A12, 1:100; RRID:AB_532379), rabbit anti-CHGA (Abcam, Cambridge, UK; ab68271, 1:500; RRID:AB_11154750), and rat anti-CPR (DSHB; GN-ID4, 1:200; RRID:AB_2255626). .. Primary antibodies were diluted with blocking solution and incubated at 4 ◦C for 16 h. The primary antibodies used for the flow cytometry analysis included goat anti-SOX17 (R&D Systems; AF 1924, 1:200; RRID:AB_355060), mouse anti-HNF4α (Santa Cruz Biotechnology; sc-374229, 1:500; RRID:AB _10989766), goat anti-PDX1 (R&D Systems; AF 2419, 1:200; RRID: AB_355257), mouse anti-NKX6.1 (DSHB, Iowa City, IA, USA; F55A12, 1:100; RRID:AB_532379), rabbit anti-CHGA (Abcam, Cambridge, UK; ab68271, 1:500; RRID:AB_11154750), and rat anti-CPR (DSHB; GN-ID4, 1:200; RRID:AB_2255626).

    Immunocytochemistry:

    Article Title: Depolymerizing F-actin accelerates the exit from pluripotency to enhance stem cell-derived islet differentiation.
    Article Snippet: After 24 hours, the slides were incubated with secondary antibodies for 2 hours at room temperature and then finally sealed with DAPI Fluoromount-G (0100-20, SouthernBiotech). .. Primary antibodies were diluted in ICC solution at 1:300 unless otherwise indicated: rat anti-C-peptide (GN-ID4-S, Developmental Studies Hybridoma Bank (DSHB)), 1:100 mouse anti-NKX6-1 (F55A12-S, DSHB), goat anti-PDX1 (AF2419, R&D Systems), mouse anti-SOX17 (MAB1924, R&D Systems), mouse anti-GCG (H00002641, Abnova), 1:250 mouse anti-SST conjugated to Alexa Fluor 488 (566032, BD Biosciences), AR TI CL E IN P RE SS rabbit anti-FOXA2 (07-633, MilliporeSigma) or (ab108422, Abcam), mouse anti-OCT-3/4 (sc5279, Santa Cruz Biotechnology), goat anti-NANOG (AF1997, R&D Systems), rabbit anti-Lefty (ab22569, Abcam), mouse anti-Nodal (ab55676, Abcam), rabbit anti-c-Jun (ab40766, Abcam), rabbit anti-TAGLN (ab14106, Abcam), rabbit anti-beta catenin (ab32572, Abcam), mouse antiOTX2 (NBP2-37597, Novus Biologicals), sheep anti-ONECUT1 (AF6277, Novus Biologicals), rabbit anti-BRACHYURY (D2Z3J, Cell Signaling Technology), and rabbit anti-PAX6 (D3A9V, Cell Signaling Technology). .. F-actin was stained directly with 1:500 TRITC-conjugated phalloidin (FAK 100, MilliporeSigma).

    Article Title: Depolymerizing F-actin accelerates the exit from pluripotency to enhance stem cell-derived islet differentiation.
    Article Snippet: The cells were AR TI CL E IN P RE SS washed once with PBS and then run on a Cytek Northern Lights flow cytometer (Cytek Biosciences). .. Primary antibodies were added to ICC solution at the indicated dilutions: 1:300 rat anti-C-peptide (GN-ID4-S, Developmental Studies Hybridoma Bank (DSHB)), 1:100 mouse anti-NKX6-1 (F55A12-S, DSHB), 1:300 goat anti-PDX1 (AF2419, R&D Systems), 1:1,000 rabbit anti-CHGA (ab15160, Abcam), 1:250 mouse anti-SST conjugated to Alexa Fluor 488 (566032, BD Biosciences), 1:350 mouse anti-GCG conjugated to BV421 (565891, BD Biosciences), 1:300 rabbit anti-SLC18A1 (HPA063797, MilliporeSigma), 1:625 mouse anti-FOXA2 conjugated to PE (561589, BD Biosciences), 1:625 anti-SOX17 conjugated to APC (130-111-033, Miltenyi Biotech). ..



    Similar Products

    93
    R&D Systems goat anti pdx1
    Goat Anti Pdx1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+pdx1/Human+PDX-1%2FIPF1+Antibody/pm41927580-280-25-28
    Average 93 stars, based on 1 article reviews
    goat anti pdx1 - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    R&D Systems d16g10 goat anti pdx1 r d systems rrid ab 10083456
    D16g10 Goat Anti Pdx1 R D Systems Rrid Ab 10083456, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+pdx1/Human+PDX-1%2FIPF1+Antibody/pm41385368-469-36-39
    Average 93 stars, based on 1 article reviews
    d16g10 goat anti pdx1 r d systems rrid ab 10083456 - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    96
    Jackson Immuno goat anti pdx1
    Goat Anti Pdx1, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+pdx1/Cy+3+AffiniPure+Donkey+Anti-Rabbit+IgG/pm40485586-144-20-48
    Average 96 stars, based on 1 article reviews
    goat anti pdx1 - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    Image Search Results